Let's try and fix your 16-bit NT system to stop the error
C:\WINDOWS\SYSTEM32\AUTOEXEC.NT. This is caused by corrupt system files. hence the "16 bit MS-DOS Subsystem" message.
Download
xp_fix.exe and run it. Reboot your computer when finished. Now when you run the application file you should not get this error message.
Additional information about this error and how to fix it manually can be found here:
http://support.microsoft.com/default...b;en-us;324767